I. General information |
Synonyms: |
COLO-824 |
Background: |
established from the pleural fluid of a 52-year-old woman with breast carcinoma in 1991 |
Species: |
human (Homo sapiens) |
Tissue: |
breast |
Disease: |
breast carcinoma |
Gender: |
N/A |
Morphology: |
cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension |
Growth Mode: |
N/A |
Doubling Time: |
ca. 60-80 hours |
DNA Profile: |
N/A
Our Cell Line Authentication Service |
Culture Medium: |
RPMI-1640+15%FBS
COLO-824完全培養基,# CBP60362M
We strongly suggest to purchase the complete medium from us.
|
Cryopreservation medium: |
90%FBS+10%DMSO |
Mycoplasma: |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
Fingerprint: |
multiplex PCR of minisatellite markers revealed a unique DNA profile |
Species: |
confirmed as human with IEF of AST, MDH, NP |
Cytogenetics: |
human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?) - carries small bisatellited marker |
Virus |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
Comments: |
For more information, please contact us (4008-750-250). |